22.03.04 Nws
KhoomAbstract
DYRK1A yog ib hom kab mob loj hauv Down syndrome (DS).Txo qhov tshwm sim ntawm cov qog nqaij hlav xws li neuroblastoma hauv DS cov neeg mob thiab nce vascular anomalies hauv DS fetuses qhia txog lub luag haujlwm ntawm DYRK1A hauv cov txheej txheem angiogenic, tab sis hauv vivo pov thawj tseem tsis tshua muaj.
Ntawm no, cov kws tshawb fawb siv zebrafish dyrk1aa mutant embryos kom nkag siab txog DYRK1A muaj nuj nqi hauv kev tsim cov hlab ntsha hlwb.Zebrafish dyrk1aa mutants nthuav tawm cerebral hemorrhage thiab tsis xws luag nyob rau hauv angiogenesis ntawm central hlab ntsha nyob rau hauv lub hindbrain tsim.Ua ke, cov txiaj ntsig tau qhia tias DYRK1A ua lub luag haujlwm tseem ceeb hauv angiogenesis thiab kev saib xyuas ntawm kev txhim kho cerebral vasculature los ntawm kev tswj hwm ntawm calcium signaling, uas yuav muaj peev xwm kho tau rau DYRK1A-txog cov kab mob vascular.
Fig1 dyrk1ab yog qhia nyob rau hauv cov cheeb tsam ntawm lub hlwb.Los ntawm WISH (tag nrho mount nyob rau hauv situhybridization), dyrk1ab tau qhia nyob rau hauv lub forebrain (dub xub xub taub hau, af; dub brackets, gl), lub midbrain (grey arrowheads, af; grey brackets, gl), lub hindbrain (xiav xub xub taub hau, af; xiav brackets, gl) ntawm 24, 48 thiab 72 hpf thiab qaum qaum (txiv kab ntxwv xub taub hau, a thiab b) ntawm 24 hpf.Nws kuj tau kuaj pom hauv lub plawv (asterisks, d, f, j thiab l) thiab hauv retina (xov liab, il) ntawm 48 thiab 72 hpf.(m thiab n) Sectioned dluab ntawm WISH embryos qhia tau hais tias dyrk1ab nyob rau hauv lub tectum (ntsuab xub) thiab lub retina (xov liab) ntawm 48 hpf thiab 72 hpf.
Kev tsom xam ntawm kev siv technology
Qhov kev sim yuav tsum tau ua kom pom cov noob caj noob ces ntawm zebrafish, uas yuav tsum tau lub koob yees duab kom muaj qhov zoo rhiab heev los txo lub sij hawm raug thiab qhov loj txaus ntawm kev pom los sau cov ntaub ntawv ntxiv rau qhov kev sim.Readout suab ntawmDhyana 400 DClub koob yees duab tsuas yog 2 electrons.Cov nyhuv txias txias ntawm -10 ℃ tuaj yeem tswj tau qhov tsaus ntuj tam sim no thiab txhim kho cov teeb liab-rau-suab nrov piv.1.2 "muab thaj chaw loj dua rau cov neeg saib xyuas lub tshuab tsom, txo cov duab thiab lub sijhawm tau txais, thiab muab kev txhawb nqa zoo rau kev tshawb fawb.
Kev siv qhov chaw
Cho, HJ, Lee, JH, Kim, JH, Kim, SY, Huh, YH, Kim, HJ, Lee, KS, Yu, K., & Lee, JS (2019).Vascular defects ntawm DYRK1A knockouts yog ameliorated los ntawm modulating calcium signaling nyob rau hauv zebrafish.Kab mob qauv & mechanisms, 12(5), dmm037044.https://doi.org/10.1242/dmm.037044
