22.03.04 Nws
KhoomAbstract
Cov neeg laus cov qauv kuaj hauv qhov kev tshawb fawb tam sim no tau sau los ntawm thaj chaw intertidal ntawm sab qab teb thiab sab hnub poob ntawm ntug dej hiav txwv Kauslim siv 500 μm-mesh sieves.Cov kev soj ntsuam tau ua rau ob qho tib si nyob thiab cov qauv ruaj khov.Cov qauv nyob tau so hauv 10% MgCl2 tov, thiab cov yam ntxwv morphological tau pom nyob rau hauv stereomicroscope (Leica MZ125; Lub teb chaws Yelemees).Cov duab thaij tau siv lub koob yees duab digital (TucsenDhyana 400 DC;Fuzhou Fujian, Suav teb) nrog rau kev ntes (Tucsen Mosaic version 15; Fuzhou Fujian, Suav).Kev soj ntsuam morphological ntawm Spio cov qauv los ntawm sab qab teb thiab sab hnub poob ntug dej hiav txwv ntawm Kaus Lim Kauslim, ua ke nrog kev tshuaj ntsuam molecular ntawm peb thaj chaw gene los ntawm cov ntaub ntawv sau tshiab, qhia pom tias muaj ib hom tsis tau piav qhia yav dhau los ntawm Spio, S.pigmentata sp.
Fig 1 Spiopigmentata sp.nov.A, B holotype (NIBRIV0000888168), tsau hauv formalin C, D paratype (NIBRIV0000888167), tsau hauv formalin A anterior kawg, dorsal saib B anterior kawg, ventral saib C methyl ntsuab staining qauv ntawm anterior kawg, ventral saib, ) D neuropodial hooded hooks los ntawm chaetiger 15, inconspicuous uppermost hniav ( xub).Scale bars: 0.5 hli (A-C);20.0m d ib.
Kev tsom xam ntawm kev siv technology
Kev tshawb pom ntawm hom tshiab yuav tsum tau ua tib zoo saib xyuas morphological.Cov kws tshawb fawb tau tshuaj xyuas hom tshiab Spio nyob rau sab qab teb thiab sab hnub poob ntug dej hiav txwv ntawm Kaus Lim Qab Teb.Dhyana 400 DCLub koob yees duab tau siv rau kev soj ntsuam, raws li cov xim tsis tshua muaj sCMOS lub koob yees duab ntawm kev ua lag luam, nws 6.5 μm pixel tuaj yeem phim qhov kev daws teeb meem ntawm lub hom phiaj lub zog siab theem thiab muab cov xwm txheej rau kev nthuav tawm morphological txawv ntawm hom tshiab.
Kev siv qhov chaw
Lee GH, Mei?ner K, Yoon SM, Min GS.Cov hom tshiab ntawm genus Spio (Annelida, Spionidae) los ntawm sab qab teb thiab sab hnub poob ntug dej hiav txwv ntawm Kauslim.Zookeys.2021; 1070:151-164.Published 2021 Nov 15. doi:10.3897/zookeys.1070.73847
